• A portable, cheap, point-of-care diagnostics for rapid determination of total cholesterol, HDL, LDL and triglycerides in a single run might become a possible with researchers at Centre for Cellular and Molecular Biology (CSIR-CCMB), Hyderabad successfully fabricating a paper-based microfluidic device.
  • The device has high specificity while the sensitivity is comparable with conventional methods. The device has to be validated with more blood samples.
  • The flower-shaped device with five arms is printed on a filter paper to simultaneously detect total cholesterol, HDL, LDL and triglycerides; the fifth arm acts as a control.
  • Cholesterol and triglycerides can be detected in less than eight minutes using the microfluidic device.
  • Only 10 microlitre of serum sample is needed to determine the four parameters.
  • The filter paper is functionalised with aminosilane (3-aminoproplytriethoxysilane or APTES) and gold nanoparticles.
  • The aminosilane acts as a binder to immobilise both gold nanoparticles and enzymes on the paper surface.
  • While the enzymes react with the serum and help in detecting cholesterol and triglycerides, the gold nanoparticles enhance the intensity of the detection dye to produce a visible change in colour based on the amount of cholesterol and triglycerides present in the sample.